| 名稱 | FcεRI Effector Reporter Cell |
| 型號(hào) | CBP74285 |
| 報(bào)價(jià) |  |
| 特點(diǎn) | FcεRI Effector Reporter Cell |
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地址：南京市棲霞區(qū)緯地路9號(hào)
Email： zhangxiangwen@cobioer.com
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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74285FcεRI Effector Reporter Cell
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| I. Background | |
IgE由兩條重鏈（ε鏈）和兩條輕鏈組成，F(xiàn)c段含高親和力受體（FcεRI）結(jié)合位點(diǎn)，半衰期僅2-3天（IgG為21天）。作為免疫系統(tǒng)的“哨兵"，IgE既可結(jié)合寄生蟲(chóng)表面抗原，激活嗜酸性粒細(xì)胞釋放毒性顆粒（如主要堿性蛋白），通過(guò)ADCC（抗體依賴細(xì)胞介導(dǎo)的細(xì)胞毒性）清除寄生蟲(chóng)（如血吸蟲(chóng)、鉤蟲(chóng)）；也能因過(guò)度反應(yīng)引發(fā)致命過(guò)敏；IgE還可靶向腫瘤抗原（如HER2），激活巨噬細(xì)胞ADCC效應(yīng)；并且最近有研究指出，腦膜肥大細(xì)胞通過(guò)IgE-MRGPRX2可加劇阿爾茨海默病病理。 | |
| II. Description | |
FcεRI Effector Reporter Cell 藥靶模型很好的模擬了 FcεRI的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程，原理見(jiàn)下圖所示。
Figure 1. FcεRI Effector Reporter Cell細(xì)胞模型原理圖 | |
| III. Introduction | |
| Expressed gene: | FcεRI |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640+10%FBS+ 1 μg/ml Puromycin+ 10 μg/ml Blasticidin + 800 μg/ml Hygromycin B+ 1000 μg/ml G418 |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data
Figure 2. Recombinant FcεRI Effector Reporter Cell stably expressing FcεRIA.
Figure 3. Dose Response of Human IgE in FcεRI Effector Reporter Cell(C4).
Figure 4. Inhibition of Human IgE-induced Reporter Activity by Human IgE Neutralization Ab in FcεRI Effector Reporter Cell(C4). | |
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