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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74009CD40/NFkB-Luc/HEK293

名稱	CD40/NFkB-Luc/HEK293
型號(hào)	CBP74009
報(bào)價(jià)
特點(diǎn)	CD40/NFkB-Luc/HEK293，凍存條件：90% FBS+10% DMSO

詳細(xì)內(nèi)容

I. Background
CD40 是腫瘤壞死因子受體超家族（TNFRSF）成員，作為免疫調(diào)節(jié)的關(guān)鍵分子，廣泛參與 B 細(xì)胞活化、抗原呈遞細(xì)胞（APC）成熟及 T 細(xì)胞免疫應(yīng)答。其配體 CD40L（CD154）主要表達(dá)于活化的 T 細(xì)胞，通過(guò) CD40-CD40L 互作橋接先天性與適應(yīng)性免疫，是治療、自身免疫病及感染性疾病的熱門靶點(diǎn)。 CD40 信號(hào)轉(zhuǎn)導(dǎo)主要利用 TNF 受體相關(guān)因子的銜接蛋白，導(dǎo)致經(jīng)典和非經(jīng)典 NFκB 通路、MAPK、PI3K 和 PLCγ的激活。它們的激活導(dǎo)致特征性下游效應(yīng)，包括轉(zhuǎn)錄激活、細(xì) 胞骨架重排和細(xì)胞存活。

II. Description
CD40 NFκB-Luc HEK293 報(bào)告基因藥靶模型很好的模擬了體內(nèi) CD40 的信號(hào)轉(zhuǎn)導(dǎo)過(guò)程，原理見下圖所示。 Figure 1. CD40 NFκB-Luc HEK293 細(xì)胞模型原理圖

III. Introduction
Host Cell:	HEK293
Expressed gene:	CD40
Stability:	32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.)
Freeze Medium:	90% FBS+10% DMSO
Culture Medium:	MEM+10%FBS+1% NEAA+1 mM NaP+1 μg/ml Puromycin+100 μg/ml Hygromycin B
Mycoplasma Testing:	Negative
Storage:	Liquid nitrogen
Application(s):	Functional(Report Gene) Assay

IV. Description of Host Cell Line
Organism:	Human
Tissue:	kidney
Morphology:	Epitheloid cell
Growth Properties:	Adherent

Ⅴ. Representative Data
Figure 2. Recombinant CD40/NFκB-Luc/HEK293 constitutively expressing CD40.
Figure 3. Dose Response of Human CD40L in CD40 NFκB-Luc HEK293( C4). Figure 4. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Blocking of hCD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293(C4). Blocking of CD40L induced Activity by CD40L Blocking Ab in CD40 NFκB-Luc HEK293 Cell(C4) with CD40L Target Cell(C7).